Proteomics Today: Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology (Wiley - Interscience Series on Mass Spectrometry)

Book Description

The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.
* Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome
* Explains the hows and whys of using mass spectrometry in proteomic analysis
* Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis
* Comments on future challenges and how they may be addressed
* Includes sections on troubleshooting

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"The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.

Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome
Explains the hows and whys of using mass spectrometry in proteomic analysis
Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis
Comments on future challenges and how they may be addressed
Includes sections on troubleshooting

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Protein Microarrays
Mark Schena
Manufacturer: Jones and Bartlett Publishers, Inc.
ProductGroup: Book
Binding: Hardcover

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ASIN: 0763731277

Book Description

Protein Microarrays is one of the first books in this exciting new technology and will be an essential reference for most biologists and biotechnology professionals.

Exploration and Analysis of DNA Microarray and Protein Array Data (Wiley Series in Probability and Statistics)

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Excellent!

Exploration and Analysis of DNA Microarray and Protein Array Data (Wiley Series in Probability and Statistics)
Dhammika Amaratunga , and Javier Cabrera
Manufacturer: Wiley-Interscience
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Binding: Hardcover

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ASIN: 0471273988

Book Description

The emergence of genomics, the study of genes, is one of the major scientific revolutions of this century. Microarrays, a method used to analyze numerous DNA samples rapidly, enables scientists to make sense of this mountain of data using statistical analysis. They are being used in such areas of biomedical research as studying patterns for gene activity that cause cancers to spread. This book presents a comprehensive methodology for analyzing DNA microarray and protein array data.
The most comprehensive treatment of this important emerging field, Exploration and Analysis of DNA Microarray and Protein Array Data includes:
A review of basic molecular biology and a chapter introducing microarrays and their preparation
Chapters on processing scanned images, preprocessing microarray data, group comparative experiments, and other designs
Discussions of clustering, protein arrays, and applications for diagnostic tools
Ample exercises assist absorbtion

Customer Reviews:

Excellent!.......2004-03-30

This book provides an excellent overview of various methods in DNA microarray analysis. It explains most of the theories behind the algorithms, so that you know why the analyses are done in certain way. In fact, I find I get more insights from the book as compare to the research papers which tend to be brief.

Peptide Arrays on Membrane Supports: Synthesis and Applications (Springer Lab Manuals)

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Peptide Arrays on Membrane Supports: Synthesis and Applications (Springer Lab Manuals)

Manufacturer: Springer
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Binding: Spiral-bound

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ASIN: 3540425322

Book Description

Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.
The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.

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The other reviewer is right about the misleading title
Highly Recommended
Recommended Book
A grand introduction to a daunting field
Excellent Foundational Text on Microarray Technology

Microarray Analysis
Mark Schena
Manufacturer: Wiley-Liss
ProductGroup: Book
Binding: Hardcover

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ASIN: 0471414433

Book Description

This authoritative text begins with an introduction to basic microarray technology. The author then provides clear explanations of the conceptual and theoretical basis of this technology, followed by thorough and multi-disciplinary coverage of modern and emerging applications. The coverage includes chapters on microarray informatics, gene expression profiling, genetic diagnostics, and novel microarray technologies.

Customer Reviews:

The other reviewer is right about the misleading title.......2005-09-10

Despite the fact that this book has very little about microarray data analysis, it is still a very good book about microarray technology. The author even introduces the reader to the basic organic chemistry and the physics necessary for fully understanding what is a microarray and how it works. The book has many interesting information which I did not see in other similar books and which can help you create the necessary microarray culture you will need. On the other hand, the book does not provide protocols or detailed methods for your experiments (if you are looking for something like this you will need the "DNA MICROARRAYS: A Molecular cloning manual" which is some kind of Maniatis of the microarrays).

In relation to data analysis this book does not provide you with even the basics for analysing your data, which is a fairly complex task. If you need help with that (and you are a biologist) I would suggest "Data analysis tools for DNA Microarrays" by Sorin Draghici, which is a very good intro book to data analysis and is not too hard on the math side (actually it is as simple as possible but without oversimplifying it). If you are a math person than you might prefer Terry Speed's "Statistical Analysis of Gene Expression Microarray Data" or Cabrera's "Exploration and Analysis of DNA Microarray and Protein Array Data".

Highly Recommended.......2003-11-01

"...the book is highly recommended as an excellent one stop resource on microarray technology" (Proteomics)

Recommended Book.......2003-07-25

"...a very personal, encyclopedic, diffuse compendium of everything known about microarrays...fun to read...full of information that a working biologist...might be quite interested to learn." (ASM News, Vol. 69, No. 7, 2003)

A grand introduction to a daunting field.......2003-07-01

As an emerging discipline, Microarray analysis is somewhat overwhelming and this book provides an excellent overview and introduction. While it does not give the reader the ability to perform high level data analysis, it certainly provides the criteria which would be necessary to design one. I personally rather enjoyed the casual and light hearted editaorial handling of what could have been a very dry text. I am an engineer, interested in the techniques of Microarray analysis more than the actual execution, but even I found the book extremely valuable. An excellent introductory and intermediate text. Readable by and valuable to almost anyone with a basic background in and understanfding of the life sciences. Extraordinary and a great addition to any library!

Excellent Foundational Text on Microarray Technology.......2003-06-22

The Microarray Analysis book by Dr. Mark Schena does an excellent job of taking the reader thru the multiple technologies and science disciplines required to understand and use the power of the microarray. It's interdisciplinary and broad approach allows complete understanding of the field without wasting time on aspects of the field which are sill evolving. It's elegantly written. Chapter 13, which talks about the use of microarrays in genetic screening, testing and diagnostics, is one of Schena's greatest accomplishments, in a historic and socio-economic sense. This contribution alone, makes the book a must read to prepare for the microarray revolution in medicine, agriculture, ecology and bio-threat detection. Strongly recommended reading for anyone seriously interested in Microarrays, be they students, professors expanding their horizons or CEO's hoping to mine microarrays. Hats off to Dr. Mark Schena!

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Focuses on surface plasmon resonance

Protein Microarray Technology

Manufacturer: Wiley-VCH
ProductGroup: Book
Binding: Hardcover

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Protein Microarrays

ASIN: 3527305971

Book Description

This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins.

The list of authors consisting of world leading experts provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions.

Topics covered include:

Types of biomolecular interactions
Surface chemistry
Detection technologies
Spotting technologies
Bioinformatics/data analysis

While primarily intended to serve as a reference for researchers and students embarking on the exciting fields of proteomics, drug discovery and clinical diagnostics, this technology is also expected to potentially impact the areas of food diagnostics, environmental monitoring and national security.

Customer Reviews:

Focuses on surface plasmon resonance.......2005-10-26

In support of this book it was pretty clear and did a very good job citing relevant papers and companies producing the technology. I'm not saying it's good or bad but this book focuses heavily on surface plasmon resonance (SPR) detection of analyte. By this I mean about 1/3 of its ~230 pages are on SPR. SPR has its own advantages and disadvantages but if you're not planning to use it, this probably isn't the book for you. Check out Schena's Protein Microarrays if you're looking for less specific coverage (about twice as many pages and cost me 1/4 the price).

Applying Genomic and Proteomic Microarray Technology in Drug Discovery

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Practical Reference On Microarray Analysis

Applying Genomic and Proteomic Microarray Technology in Drug Discovery
Robert S. Matson
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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ASIN: 0849314690

Book Description

Microarrays play an increasingly significant role in drug discovery. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. The author addresses the drawbacks, helping you avoid unnecessary pitfalls, and provides practical tips on how to employ the technology in drug discovery and development. The book details the commercial landscape, covering the many issues surrounding the future adoption of gene expression and protein microarrays for pharmacogenomic and pharmacoproteomic applications. The author critically assesses those studies that have helped define applications in genomics and proteomics, explains gene expression microarray applications, and examines the utility of the protein microarray. He covers alternative substrates and the preparation of various surface chemistries together with their suitability for immobilization of nucleic acids and proteins. He delineates the mechanics of microarraying including environmental conditions, printer and pin performance, as well as discussion regarding setting up the print run. The book supplies protocols for printing nucleic acids and proteins and an in-depth discussion of other important parameters such as print buffers (inks) and factors influencing print quality. An understanding of the making of a microarray is fundamentally important to those interested in producing "spotted" arrays and their proper use. As this technology expands in popularity and usefulness, industry experts must grasp the fundamental principles behind it, its strengths, and its limitations. A basic reference for users of microarray technology in drug discovery, this book offers a detailed perspective and insight into the present and future uses of this technology.

Customer Reviews:

Practical Reference On Microarray Analysis.......2005-01-08

This book details the commercial array landscape, covering the many issues surrounding the future adoption of gene expression and protein microarrays for pharmacogenomic and pharmacoproteomic applications. The author critically assesses those studies that have helped define applications in genomics and proteomics, explains gene expression microarray applications, and examines the utility of the protein microarray.
An understanding of the process used in making microarrays is fundamentally important to those interested in producing "spotted" arrays and using them properly. As this technology expands in popularity and application, industry experts must grasp the fundamental principles behind it, its strengths, and its limitations. A basic reference on the benefits of microarray technology in drug discovery, this publication offers a detailed perspective and insight into the present and future uses of this technology.
Features
Includes an extensive literature survey and comparison of microarray technology formats
Discusses the relevance and general utility of microarray technologies in the drug discovery process
Provides an in-depth discussion of important factors for successful array printing Contains protocols for printing nucleic acids and proteins and a selection of substrates and preparation of surface chemistries
Supplies an extensive review and assessment of key studies demonstrating the utility of gene expression and protein microarrays
Array technology, much like polymerase chain reaction (PCR) technique, was created to satisfy an existing need in molecular biology. PCR provided a means to amplify enough DNA to sequence genes. The first applications for arrays involved gene sequencing by hybridization (SBH) and genotyping. However, gel-based sequencing quickly supplanted the emerging SBH approach, while genotyping and mutation analysis have been slow in development. The challenge for those involved in array technology then became finding that elusive application niche, one that would demonstrate a clear, unmitigated, and thereby sustained need for the technology.
This book picks up the array technology journey from the mid-1990s with the introduction of microarray-based gene expression analysis. The global analysis of genes by microarrays has provided a fresh and exciting view of the cellular process. More importantly, it enabled others to consider similar utility in various "omic" fields. Hence, we have witnessed the emergence of protein arrays to address proteomics.
In writing this book, my aim was first to provide a detailed description and offer insight into present and future utilities for microarray technology. While arguably array-based technologies are now being adopted in diverse fields, I have placed emphasis on applications related to drug discovery. Microarrays continue to play significant and increasingly important roles in the drug discovery process.
Chapter 1 considers the respective roles as well as the many issues surrounding the future adoption of gene expression and protein microarrays for pharmacogenomic and pharmacoproteomic applications. For acceptance by the pharmaceutical and diagnostic industries, commercially validated array technology is required. Chapter 2 details the commercial microarray landscape. Chapter 3 describes alternative substrates and the preparation of various surface chemistries along with their suitability for immobilization of nucleic acids and proteins. In Chapter 4, the mechanics of microarraying are described in detail including environmental conditions, printer and pin performance, and instructions for setting up a print run. Protocols for printing nucleic acids and proteins are provided along with in-depth discussion of other important parameters such as print buffers (inks) and factors influencing print quality. I also set out to discuss the importance and provide a critical assessment of studies that helped to define applications in genomics and proteomics. In Chapter 5, gene expression microarray applications are described; Chapter 6 examines the utility of protein microarrays.
Finally, an understanding of the making of a microarray is fundamentally important to those interested in producing "spotted" arrays and properly using them. While complementary (cDNA) microarray fabrication on glass slides has been well studied, we have less experience with the attachment of oligonucleotides and the preparation of protein arrays. Moreover, additional substrates and surface chemistries that may be better suited for printing proteins are now available.
Robert (Bob) Matson, Ph.D., is a senior staff scientist in the Advanced Technology Center at Beckman Coulter, Inc., Fullerton, California. He has been involved in the development of both nucleic acid and protein array-based technology for the past 13 years. His initial introduction to array technology began in collaboration with Sir Edwin Southern in developing an in situ oligonucleotide array synthesis platform for the corporation. Later work by Dr. Matson and his research team produced some of the
first plastic microplate-based microarrays. Beckman Coulter recently launched the A2TM plate based upon the microplate "array of arrays" concept.
Prior to joining Beckman Coulter, he served in several technical management roles including: R&D director at BioProbe International, R&D director at Costar-Nuclepore, and R&D group leader, chemistry, at BioRad Lab-oratories.
Dr. Matson currently holds seven United States patents and has contributed numerous papers in peer-reviewed journals as well as chapters in several books on microarrays. He has also made many presentations in the United States and abroad on the development of microarray technology. His current interest is in automated approaches to multiplexed assay development.
Dr. Matson grew up in the San Juan Islands of Washington State and attended Western Washington University, Bellingham, where he earned his B.A. and M.S. in chemistry. He received his Ph.D. in biochemistry from Wayne State University. Following postdoctoral studies at the medical school of the University of California at Los Angeles, he served as a principal investigator with the Veterans Administration Medical Center and as an adjunct professor of biological chemistry at the medical school of the University of California at Davis. Dr. Matson also held a faculty lectureship in the department of chemistry at University of Southern California and was an assistant professor of chemistry at the University of Southern Maine, Portland. He served on the editorial boards of Applied Biochemistry and Biotechnology and the Journal of Preparative Chromatography, and is a member of the Scientific Advisory and Organizing Board of International Business Compunications' "Chips to Hits" conferences.

Expression Genetics: Accelerated and High-Throughput Methods (Biotechniques Update Series)

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Expression Genetics: Accelerated and High-Throughput Methods (Biotechniques Update Series)

Manufacturer: Eaton Publishing Company/Biotechniques Books
ProductGroup: Book
Binding: Paperback

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Book Description

This book continues the Expression Genetics theme and is intended to complement the previous volume on differential display. The methods included here, e.g., mammalian expression vectors, would come into play once regulation of a gene has been discovered by differential display. Advances in alternatives to differential display are also presented, including AFLP, arrays, protein display, and the two-hybrid system. These 37 papers selected from the journal BioTechniques along with their accompanying updates emphasize technologies that accelerate screening and selection assays.

Functional Protein Microarrays in Drug Discovery (Drug Discovery Series)

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Functional Protein Microarrays in Drug Discovery (Drug Discovery Series)

Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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ASIN: 0849398096

Book Description

An ideal text for biotechnologists, protein chemists, and biochemists, Functional Protein Microarrays in Drug Discovery explores all aspects of functional protein microarrays, including basic principles, methods, and applications. The book discusses the generation of functional protein content and describes both standard and state-of-the-art fabrication methods. It reviews current and next generation approaches to assay detection and presents a wide range of applications, from biomolecular interaction discovery and characterization to immune response profiling. The book also addresses several fundamental computational issues, bioinformatics anddata analysis.

Microarray Technology and Its Applications (Biological and Medical Physics, Biomedical Engineering)

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Microarray Technology and Its Applications (Biological and Medical Physics, Biomedical Engineering)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 3540229310

Book Description

The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has since been emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved.

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